LOCUS       sequence-165545-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mobilisable shuttle and expression vector. Replicates in many 
            Gram-negative bacteria. Has multiple cloning site with blue/white 
            selection function. Cloned genes driven by derepressed lac 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM 2nd, 
            Peterson KM
  TITLE     Four new derivatives of the broad-host-range cloning vector 
            pBBR1MCS, carrying different antibiotic-resistance cassettes.
  JOURNAL   Gene. 1995 Dec 1;166(1):175-6.
  PUBMED    8529885
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1995-12-1"; volume: "166(1)"; pages: "175-6"
COMMENT     Sequence Label: pBBR1MCS-2
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..113
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     30..46
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     30..46
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(80..96)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(81..97)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(126..146)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(126..144)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(165..181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(165..181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(178..200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(213..219,220..237,238..243))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    258..279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(795..814)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 aattggagct ccaccgcggt ggcggccgct ctagaactag tggatccccc gggctgcagg
       61 aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt acccagcttt
      121 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
      181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      241 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      301 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      361 agaggcggtt tgcgtattgg gcgcatgcat aaaaactgtt gtaattcatt aagcattctg
      421 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      481 ttgtcgcctt gcgtataata tttgcccatg ggggtgggcg aagaactcca gcatgagatc
      541 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
      601 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
      661 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
      721 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
      781 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
      841 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
      901 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
      961 ctggcgaaca gttcggctgg cgcgagcccc tg
//
