LOCUS       sequence-165547-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mobilisable shuttle and expression vector. Replicates in many 
            Gram-negative bacteria. Has multiple cloning site with blue/white 
            selection function. Cloned genes driven by derepressed lac 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM 2nd, 
            Peterson KM
  TITLE     Four new derivatives of the broad-host-range cloning vector 
            pBBR1MCS, carrying different antibiotic-resistance cassettes.
  JOURNAL   Gene. 1995 Dec 1;166(1):175-6.
  PUBMED    8529885
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1995-12-1"; volume: "166(1)"; pages: "175-6"
COMMENT     Sequence Label: pBBR1MCS-2
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    23..130
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     39..55
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     40..56
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(90..106)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(90..106)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(138..157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(139..157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(167..184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(167..183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
       61 gcttgatatc gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc
      121 ggtggagctc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
      181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      301 tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt
      361 aaatttttgt taaatcagct cattttttaa ccaataggcc gtactgcgat gagtggcagg
      421 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct
      481 gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg acccggtcgt
      541 cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta ccggtttatt
      601 gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc
      661 tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc agagcctgat
      721 aaaaacggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg
      781 ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gaggcggcta
      841 cagccgatag tctggaacag cgca
//
