LOCUS       sequence-165550-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral construct to express dCas9 fused with the catalytic 
            domain of Tet1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, 
            Young RA, Jaenisch R
  TITLE     Editing DNA Methylation in the Mammalian Genome.
  JOURNAL   Cell. 2016 Sep 22;167(1):233-247.e17. doi: 
            10.1016/j.cell.2016.08.056.
  PUBMED    27662091
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "233-247.e17"
COMMENT     Sequence Label: Fuw-dCas9-Tet1CD
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..78
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             82..102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             106..126
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctttacgaaa ctcggattga cctctcacag ctcggagggg atggtggcgg aggctcgcca
       61 aaaaagaaga gaaaggtaga cccaaagaaa aaacgaaaag tagatccgaa aaagaagagg
      121 aaggtgggat ccctgcccac ctgcagctgt cttgatcgag ttatacaaaa agacaaaggc
      181 ccatattata cacaccttgg ggcaggacca agtgttgctg ctgtcaggga aatcatggag
      241 aataggtatg gtcaaaaagg aaacgcaata aggatagaaa tagtagtgta caccggtaaa
      301 gaagggaaaa gctctcatgg gtgtccaatt gctaagtggg ttttaagaag aagcagtgat
      361 gaagaaaaag ttctttgttt ggtccggcag cgtacaggcc accactgtcc aactgctgtg
      421 atggtggtgc tcatcatggt gtgggatggc atccctcttc caatggccga ccggctatac
      481 acagagctca cagagaatct aaagtcatac aatgggcacc ctaccgacag aagatgcacc
      541 ctcaatgaaa atcgtacctg tacatgtcaa ggaattgatc cagagacttg tggagcttca
      601 ttctcttttg gctgttcatg gagtatgtac tttaatggct gtaagtttgg tagaagccca
      661 agccccagaa gatttagaat tgatccaagc tctcccttac atgaaaaaaa ccttgaagat
      721 aacttacaga gtttggctac acgattagct ccaatttata agcagtatgc tccagtagct
      781 taccaaaatc aggtggaata tgaaaatgtt gcccgagaat gtcggcttgg cagcaaggaa
      841 ggtcgaccct tctctggggt cactgcttgc ctggacttct gtgctcatcc ccacagggac
      901 attcacaaca tgaataatg
//
