LOCUS       sequence-165555-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For methylating EcoRI recognition sites in order to prevent cleavage
            by the EcoRI restriction enzyme. AmilCP is a blue reporter for 
            Methylase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 97 to 131)
  AUTHORS   Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, 
            Zimmermann SA, Brasino M, Rokicki J, Dowell RD
  TITLE     Engineered calcium-precipitable restriction enzyme.
  JOURNAL   ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m.
  PUBMED    25524101
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Synth Biol"; date: "2014-12-19- 19"; volume: "3"; issue: "12"; 
            pages: "969-71"
COMMENT     Sequence Label: pSB1A3 EcoRI Methylase-AmilCP
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..25
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(29..72)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    75..96
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        97..131
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
       61 aaggatgatt tctggaattc gcggccgctt ctagagttga cggctagctc agtcctaggt
      121 acagtgctag ctactagaga aagaggagaa atactagatg gccggcgcac gcaacgcgac
      181 caataagctc ctgcacaaag cgaaaaaaag caaaagcgat gagttttata cccagtattg
      241 cgacattgaa aacgaattac aatactaccg cgaacatttc agcgataagg ttgtgtactg
      301 caactgtgat gacccgcgtg tgagcaattt ttttaaatat ttcgcggtga actttgacaa
      361 cctcggactg aagaagctga ttgcaagctg ttatgtagaa aacaaggagg gctttagctc
      421 cagcgaagca gcaaaaaatg gcttttacta tgaataccat aaagaaaacg gcaaaaaact
      481 ggtcttcgat gatatttcgg tgagtagctt ctgtggcgac ggagattttc ggagcagtga
      541 aagcattgat ctgctcaaaa aaagtgatat cgtggtcacg aacccgcctt tttcactctt
      601 tcgtgaatac ttagatcaac ttattaaata cgataaaaag ttccttatca tcgccaatgt
      661 aaacagcatt acttataaag aagtgtttaa tctgatcaaa gaaaacaaga tctggctggg
      721 agtacattta ggccggggtg tgagtggctt catcgttcca gaacattacg aactgtacgg
      781 caccgaggcc cgcattgatt caaatggaaa ccgtattatc agcccgaata actgtctgtg
      841 gctgacgaac cttgacgtct tcattcgcca caaagacctg ccgttaacac gtaaatact
//
