LOCUS       sequence-165558-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For methylating EcoRI recognition sites in order to prevent cleavage
            by the EcoRI restriction enzyme. AmilCP is a blue reporter for 
            Methylase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, 
            Zimmermann SA, Brasino M, Rokicki J, Dowell RD
  TITLE     Engineered calcium-precipitable restriction enzyme.
  JOURNAL   ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m.
  PUBMED    25524101
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Synth Biol"; date: "2014-12-19- 19"; volume: "3"; issue: "12"; 
            pages: "969-71"
COMMENT     Sequence Label: pSB1A3 EcoRI Methylase-AmilCP
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..30
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(54..111)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    112..132
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 acgaccgagc gcagcgagtc agtgagcgag gaagcctgca taacgcgaag taatcttttc
       61 ggttttaaag aaaaagggca gggtggtgac accttgccct tttttgccgg actgcagcgg
      121 ccgctactag tattaaccgg tggcgaccac aggtttgcgt gcaatggaaa tttcacactg
      181 ctcaaccgaa gtgtaatcct tgttgtgatt ggttacatcc agtttgcggt caacatagtg
      241 ataccctggc atcttcacag gcttctttgc cttgtaagta gttttaaatt cacacaaata
      301 gtgaccgcct ccttctaact tcagagccat aaagttgttt cctagcagca ttccatctcg
      361 tgcaaagaga cgctcagtgt tgggttccca gccctgtgtc ttcttctgca tgacaggtcc
      421 attgggagga aagttcaaac cagagaactt gacatggtag atgaaacagt tgccttggat
      481 gctggaatca ttgctgacag tacacactgc accatcttca aagttcatga tcctctccca
      541 tgtatagccc tccgggaatg actgctttac atagtcaggg atgtcttcag ggtacttggt
      601 gaatggtatg cttccgtact gacactgtgg tgataaaata tcccaagcaa atggcagagg
      661 tccgcccttg gtgacagtga gctttaccgt ctgctccccc tcgtagggct taccttttcc
      721 atcgccttcg acctcaaagt agtgtccatt gaccgtgcct gacatataaa ccttgtaggt
      781 catttgttta gcgatcacac tgccggtttt ctgtaagcgc ttatttttaa tcaggattcg
      841 aaaatatggg cactttccgt taattgatag gtctttctca tccacacctt tccggaattt
      901 aatcaattca aattgttcag gattgaattt atgcaggaag gtgatcggca cccccataac
      961 gccattataa tccagcggaa tatctc
//
