LOCUS       sequence-165566-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty concatmer vector in which 3 gRNAs can be inserted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Andersson-Rolf A, Merenda A, Mustata RC, Li T, Dietmann S, Koo BK
  TITLE     Simultaneous paralogue knockout using a CRISPR-concatemer in mouse 
            small intestinal organoids.
  JOURNAL   Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 
            10.1016/j.ydbio.2016.10.016.
  PUBMED    27983963
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 
            10.1016/j.ydbio.2016.10.016."
COMMENT     Sequence Label: 3 gRNA concatemer
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(41..60)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     434..457
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg
       61 ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc
      121 ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa agctgctatt
      181 ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga
      241 gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg
      301 gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg
      361 gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt
      421 agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg
      481 cctcccctac ccggtagaat tcggatccca ggtcctcctc tgagatcagc ttctgcattg
      541 atgccataaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta
      601 ttttaacttg c
//
