LOCUS       sequence-16557-e        1332 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICT19r
FEATURES             Location/Qualifiers
     source          1..1332
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(383..400)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(383..399)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(392..414)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(608..627)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     727..749
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(787..805)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        873..975
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttttcgaata cgtttttttg tgggcttggg tatattgttt tttaatgtta tacttgcagt
       61 cgtgaaattt gattttcaaa tttgtagaaa aatcaagaaa ataattgcaa cattcgcttg
      121 tgtcaaaaac caatttcaac aaattttcgt gtgagaaata cattaccaga aggcattttt
      181 tcacacgatt agcattttgg actactttat taaatttttg cgtgtaattt tgaattaaat
      241 tgtattatat tactacttaa aaaacaaaaa atttgaccac tgagcggatc gaacgcccaa
      301 cctttcgatc tagagtcgaa cgcgctacca ttgcgccaag cagtcatgtt attctctctt
      361 gtcgacggat ccccgggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      421 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      481 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      541 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
      601 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      661 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      721 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
      781 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      841 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      901 aaatacattc aatatgtatc cgctcatgag acaataaccc tgataatgct tcaataatat
      961 tgaaaaagga agagtatgag tatccacatt ccgngncgcc cttatnccnt ttttggcgna
     1021 ttttgcctnc nggtttngct cncccanaac gctggngaaa gtaaaaangc tgaaaatcag
     1081 ttgggtgcng agngggtaca tcgaacngga tcncaacagc ggnaaaatcc tngaaanttt
     1141 tcncccnaaa aacgtttnca agnagaaccc ttttnaagtt cgctangggg gnnggatttn
     1201 ccntatgngc ccgggaanaa nactcggnnc ccaaancaat nccnaaaggg tggggaaanc
     1261 ccccccccca aaaacccttc ngnggggggn gaaaaaaatt gcggggcccc accnggggaa
     1321 aaccgcccna tt
//
