LOCUS       sequence-165572-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mouse PM20D1, C-term flag and his tags, in mammalian retroviral 
            expression plasmid. Amp/Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Long JZ, Svensson KJ, Bateman LA, Lin H, Kamenecka T, Lokurkar IA, 
            Lou J, Rao RR, Chang MR, Jedrychowski MP, Paulo JA, Gygi SP, Griffin
            PR, Nomura DK, Spiegelman BM
  TITLE     The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid Uncouplers
            of Mitochondria.
  JOURNAL   Cell. 2016 Jul 14;166(2):424-35. doi: 10.1016/j.cell.2016.05.071. 
            Epub 2016 Jun 30.
  PUBMED    27374330
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.071"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "424-35"
COMMENT     Sequence Label: pCLHCX-mPM20D1-flag
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(61..84)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagaggttga ttctcgagtt aatggtgatg gtgatgatgc gcggccgcgg tcttcagctc
       61 cttgtcgtca tcgtccttgt agtcggatcc gggaccggtg ggagatctgg gacgcgttag
      121 ttcatgcaga tgaggaactg gctctttgta agtgtcggca ttttggatga actcaaagat
      181 gaacttcacc tggttctggt agttctgaac ggaaactttc tcattgattc catggacacc
      241 actgaagtcc tgagggttca ggggaagggg gttgaaccgg tacatgccat tggtgatgtt
      301 ggcatagtgt cgggtgtccg tattggcaat acaaataccg gggacgacga tgtcgacttc
      361 cgggaagaca gatcgtatgg tctcttgaag cagctggtag cccatggcct ggtcatcaga
      421 ggggctgatg ggcaggggtt caaaggatct caacacatgc agctggactc tgtcatcagc
      481 cacggtgttc ttgacaagtt ctaggacctc atgtactgtc tgcgaagggt gaattcggca
      541 gttgattgta gcctgagcca atggagggat gacattcacc ttgattcctg cattgaacat
      601 ggtgagggct gtggtagttc ggaccagcgc atttgttatg gggttcctct ccattatcct
      661 gctcacaatg ggatgaaata gccacaggtt tctcaagact atattgatag ggaaggaaaa
      721 ctcatttgcc agtagcttca ttgtcttctt caatggccct cctccaaaca tattcggcat
      781 tggtgtctgc tccagtcggc tgacagcggc agaaagaatg ccaatgcttg tctcctttgg
      841 gggagctga
//
