LOCUS       sequence-16558-e        1364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOK11_34
FEATURES             Location/Qualifiers
     source          1..1364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             301..321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(411..433)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngntgnggc cttggtcgac tctagacact tttcagctac ctagaattac aattggatat
       61 ccccgcctcc caatccaccc acccagggaa aaagaagggc tcgccgaaaa tcaaagttat
      121 ctccaggctc gcgcatccca ccgagcggtt gacttctctc caccactttt cattttaacc
      181 ctcggggtac gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac
      241 atagaacatt ttcaggagga cccttcttgg agggtaccga gctcagaaaa aatgactgct
      301 ccaaagaaga agcgtaaggt accggtgggt gaagaccaga aacagcacct cgaactgagc
      361 cgcgatattg cccagcgttt caacgcgctg tatggcgaga tcgatcccgt cgttttacaa
      421 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      481 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      541 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
      601 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
      661 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
      721 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
      781 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
      841 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac
      901 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt
      961 gacggcagtt atctggaaga tcaggatatg tggcggatga ncggcatttt tccgtgacgt
     1021 ctcgttgctg cataaaccga ctacccaatc agcgatttcc atgntgccnc tcgctttang
     1081 gatgatttca ccccnctgta ctggaggctg aaatncaaat gtgcggcgag ttgcgngact
     1141 acctacgggt aanagtttct tttggcaggg nnaaancagn tcnccnnngg caccggnctt
     1201 tnggggggaa atntcnanga gnggggggtt tgccatcnct ccantcgtng aagncaaaac
     1261 cgaanggggg gcccaanncc aatnntttgg gggggtnaat ccccccccca gnccnttntn
     1321 aaaaaaccgg antctttccn ggggnaataa aagncctttc ttaa
//
