LOCUS       sequence-16559-e        1386 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICT19h
FEATURES             Location/Qualifiers
     source          1..1386
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(400..417)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(400..416)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(409..431)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(625..644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     744..766
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(804..822)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        903..993
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggcagagag ataacatgac tgcttggcgc aatggtagcg cgttcgactc tagatcgaaa
       61 ggttgggcgt tcgatccgct cagtggtcaa attttttgtt ttttaagtag taatataata
      121 caatttaatt caaaattaca cgcaaaaatt taataaagta gtccaaaatg ctaatcgtgt
      181 gaaaaaatgc cttctggtaa tgtatttctc acacgaaaat ttgttgaaat tggtttttga
      241 cacaagcgaa tgttgcaatt attttcttga tttttctaca aatttgaaaa tcaaatttca
      301 cgactgcaag tataacatta aaaaacaata tacccaagcc cacaaaaaaa cgtattctga
      361 aaaattggta tctagatctc gagctcgcga aagcttggca ctggccgtcg ttttacaacg
      421 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      481 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      541 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      601 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      661 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      721 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
      781 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      841 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaatgtgcg cggaaacccc
      901 tatttggtta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      961 ataaatgctt cataatatgg aaaaaggaag agtatgagta tccacatttc gtgtcgccct
     1021 atcccctttt ggngcatttg gccttccngt tttgctcccc cagaaccccg gngaagtaaa
     1081 anangcngaa aatcagttgg gngcccgagg ggttactcna actggatnnc acagngggaa
     1141 aatcctggna gtttcncccn naaaacgttt cnanggggaa ccctttaang ttcggcngng
     1201 ggccggnatt tccnaattgg ccnggaaaaa cncgncccna ancatttcca aagncngntg
     1261 aanccccccc naaaccntcn gggggggcaa aaaaaatgnn ggcccancgg ngaaccgggc
     1321 cntnntngaa ancgggaaaa naacccnttt tcaggggaaa tnnncttggg ngaaaaaaac
     1381 caangg
//
