LOCUS       sequence-165591-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used to express EGFP-tagged Rap1K3NR from the 
            GDP promoter in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pAG415-GPDp-EGFP-rap1-K3NR
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..72
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcggcatgga cgagctgtac aagggtggac caggtggtgg acatcaaaca agtttgtaca
       61 aaaaagcagg ctacaaaatg tctagtccag atgattttga aactgcacca gcagaatatg
      121 ttgatgcatt ggatccgagt atggtcgttg ttgacagtgg ttctgctgca gtcacagcgc
      181 catcggattc agccgctgag gtaagagcta atcaaaacga agaaaacact ggtgctactg
      241 ctgctgaaac tagtgagaaa gtggatcaga cggaggtcga aaagaaagat gatgatgata
      301 ccactgaagt cggtgtaaca actaccacgc cttccattgc tgatactgct gctaccgcta
      361 acattgcttc gacatcaggt gcatctgtta ctgaacctac tactgatgac accgctgctg
      421 atgagagaag agaacaagta agtggtcctc ctctgtcaaa tatgaaattc tatcttaatc
      481 gcgacgcgga tgcgcatgac tctttaaatg atattgatca attagcgcgt ctgattagag
      541 caaacggtgg tgaagtgcta gactccaagc caagagaatc aaaagagaat gttttcatcg
      601 tgtcacccta taatcatacc aatttaccaa cggtaactcc gacttatatt aaggcatgtt
      661 gtcaaagcaa ctcgctactg aacatggaaa attatttagt accatacgat aactttagag
      721 aagttgtcga tagtaggttg caggaggaat cgcattctaa tggtgtagat aatagcaata
      781 gcaatagcga taacaaggat tctatcaggc ccaaaacaga aataatctct acgaatacta
      841 atggcgctac ggaagactca accagcgaaa aagttatggt agacgctga
//
