LOCUS       sequence-165594-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty concatmer vector in which 4 gRNAs can be inserted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Andersson-Rolf A, Merenda A, Mustata RC, Li T, Dietmann S, Koo BK
  TITLE     Simultaneous paralogue knockout using a CRISPR-concatemer in mouse 
            small intestinal organoids.
  JOURNAL   Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 
            10.1016/j.ydbio.2016.10.016.
  PUBMED    27983963
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 
            10.1016/j.ydbio.2016.10.016."
COMMENT     Sequence Label: 4 gRNA concatemer
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(39..58)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     432..455
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_RNA        complement(513..587)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(603..851)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(661..680)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(831..851)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc
       61 gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc
      121 ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag ctgctattgg
      181 ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga
      241 ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg
      301 gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaacgga
      361 gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag
      421 cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc
      481 tcccctaccc ggtagaattc ggatccaaaa aagcaccgac tcggtgccac tttttcaagt
      541 tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaagtc ttctcgaaga
      601 ccggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt
      661 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga
      721 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta
      781 attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga
      841 aataggccct cttcctgccc gaccttggag atcccaggtc ctcctctgag atcagcttct
      901 gcat
//
