LOCUS       sequence-165596-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ABA-inducible VPR-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40...
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             578..727
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             734..754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catgggtggg ggcgcgccaa ctcaagacga attcacccaa ctctcccaat caatcgccga
       61 gttccacacg taccaactcg gtaacggccg ttgctcatct ctcctagctc agcgaatcca
      121 cgcgccgccg gaaacagtat ggtccgtggt gagacgtttc gataggccac agatttacaa
      181 acacttcatc aaaagctgta acgtgagtga agatttcgag atgcgagtgg gatgcacgcg
      241 cgacgtgaac gtgataagtg gattaccggc gaatacgtct cgagagagat tagatctgtt
      301 ggacgatgat cggagagtga ctgggtttag tataaccggt ggtgaacata ggctgaggaa
      361 ttataaatcg gttacgacgg ttcatagatt tgagaaagaa gaagaagaag aaaggatctg
      421 gaccgttgtt ttggaatctt atgttgttga tgtaccggaa ggtaattcgg aggaagatac
      481 gagattgttt gctgatacgg ttattagatt gaatcttcag aaacttgctt cgatcactga
      541 agctatgaac gaattctcaa gcgctgcagg tactagtgat gctttagacg attttgactt
      601 agatatgctt ggttcagacg cgttagacga cttcgaccta gacatgttag gctcagatgc
      661 attggacgac ttcgatttag atatgttggg ctccgatgcc ctagatgact ttgatctaga
      721 tatgctaggt agtcccaaaa agaagaggaa agtgggatcc cagtatctgc ccgacacaga
      781 tgatagacac cgaatcgaag agaaacgcaa gcgaacgtat gaaaccttca aatcgatcat
      841 gaagaaatcg cccttctcgg gtccgaccga tcccaggccc ccaccgagaa ggattgcggt
      901 cccgtcccgc tcgtcggcca gcgtgcc
//
