LOCUS       sequence-165602-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: mMaroon-RTK-mMaroon
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..785
                     /codon_start=1
                     /product="bright, rapidly maturing, monomeric far-red 
                     fluorescent protein (Bajar et al., 2016)"
                     /label=mMaroon1
                     /translation="MVSKGEELIKENMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTN
                     RIKVVRGGPLPFAFDILAPCFMYGSKTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGG
                     TLTATQDTSLQDGCLIYNVQVRGENFPSNGPVMQKKTLGWEASTETLYPADGSLEGRLD
                     WALKLVGGGHLHCRLETTYRSKKPAKNLKMPGVYFIDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gcaagggcga ggagctgatc aaggagaaca tgcacaccaa gctgtacctg acaggcaccg
      121 tgaacaacca ctacttcgaa tgcaccgccg aaggggaggg caagccctac gagggcaccc
      181 agaccaacag gatcaaggtg gtgcgcggag gccccctgcc gttcgcattc gacatcctgg
      241 ccccctgctt tatgtacggg agcaagacct tcattaacca tccacctgac attcccgatt
      301 attttaagca gtccttccct gagggcttca catgggagag aaccaccgtt tacgaagacg
      361 ggggcacact cactgctaca caggacacca gcctccagga cggctgcctg atctacaacg
      421 tccaagtcag aggggagaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
      481 gctgggaggc cagtaccgag accctgtacc ccgctgacgg cagcctggaa ggcagactgg
      541 actgggccct gaagctcgtg ggcgggggcc acctgcactg cagactggaa accacataca
      601 gatccaagaa acccgcaaag aacctcaaga tgcccggcgt ctattttatt gacaggagac
      661 tggaaagaat caaggaggcc gacaatgaga cctacgtcga gcagcacgag gtggctgtgg
      721 ccagatactg cgacctccct agcaaactgg ggcacaaact taatggcatg gacgagctgt
      781 acaagtccgg actcagatct atgggactgg aggacctcaa tatgctctac atccggcaga
      841 tg
//
