LOCUS       sequence-165603-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: mMaroon-RTK-mMaroon
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..863)
                     /codon_start=1
                     /product="bright, rapidly maturing, monomeric far-red 
                     fluorescent protein (Bajar et al., 2016)"
                     /label=mMaroon1
                     /translation="MVSKGEELIKENMHTKLYLTGTVNNHYFECTAEGEGKPYEGTQTN
                     RIKVVRGGPLPFAFDILAPCFMYGSKTFINHPPDIPDYFKQSFPEGFTWERTTVYEDGG
                     TLTATQDTSLQDGCLIYNVQVRGENFPSNGPVMQKKTLGWEASTETLYPADGSLEGRLD
                     WALKLVGGGHLHCRLETTYRSKKPAKNLKMPGVYFIDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccgctc acttgtacag ctcgtccatg ccattaagtt tgtgccccag tttgctaggg
      181 aggtcgcagt atctggccac agccacctcg tgctgctcga cgtaggtctc attgtcggcc
      241 tccttgattc tttccagtct cctgtcaata aaatagacgc cgggcatctt gaggttcttt
      301 gcgggtttct tggatctgta tgtggtttcc agtctgcagt gcaggtggcc cccgcccacg
      361 agcttcaggg cccagtccag tctgccttcc aggctgccgt cagcggggta cagggtctcg
      421 gtactggcct cccagccgag tgttttcttc tgcatcacag ggccgttgga tgggaagttc
      481 tcccctctga cttggacgtt gtagatcagg cagccgtcct ggaggctggt gtcctgtgta
      541 gcagtgagtg tgcccccgtc ttcgtaaacg gtggttctct cccatgtgaa gccctcaggg
      601 aaggactgct taaaataatc gggaatgtca ggtggatggt taatgaaggt cttgctcccg
      661 tacataaagc agggggccag gatgtcgaat gcgaacggca gggggcctcc gcgcacaacc
      721 ttgatcctgt tggtctgggt gccctcgtag ggcttgccct ccccttcggc ggtgcattcg
      781 aagtagtggt tgttcacggt gcctgtcagg tacagcttgg tgtgcatgtt ctccttgatc
      841 agctcctcgc ccttgctcac cattccg
//
