LOCUS       sequence-165604-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCyRFP1 fluorescent protein in an empty backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: mCyRFP1-C1
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..754
                     /codon_start=1
                     /product="monomeric cyan-excitable red fluorescent protein 
                     (Laviv et al., 2016)"
                     /label=mCyRFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTA
                     RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KKLKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     misc_feature    755..820
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag
       61 caagggcgag gagctgatca aggagaacat gagaagcaag ctgtacctgg aaggcagcgt
      121 gaacggccac cagttcaagt gcacccacga aggggagggc aagccctacg agggcaagca
      181 gaccgccagg atcaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc
      241 caccatgttt atgtacggga gcaaggtgtt catcaagtac cccgccgacc tccccgatta
      301 ttttaagcag tccttccctg agggcttcac atgggagaga gtcatggtgt tcgaagacgg
      361 gggcgtgctg accgccaccc aggacaccag cctccaggac ggcgagctca tctacaacgt
      421 caagctcaga ggggtgaact tcccagccaa cggccccgtg atgcagaaga aaacactggg
      481 ctgggagccc agcaccgaga ccatgtaccc cgctgacggc ggcctggaag gcagatgcga
      541 caagaagctg aagctcgtgg gcgggggcca cctgcacgtc aacttcaaga ccacatacaa
      601 gtccaagaaa cccgtgaaga tgcccggcgt ccactacgtg gaccgcagac tggaaagaat
      661 caaggaggcc gacaacgaga cctacgtcga gcagtacgag cacgctgtgg ccagatactc
      721 caacctgggc ggaggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
      781 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
      841 cataatcagc cataccacat ttgtagag
//
