LOCUS       sequence-165607-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP
            promoter in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pAG415-GPDp-EGFP-rap1-K9R
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggcatggacg agctgtacaa gggtggacca ggtggtggac atcaaacaag tttgtacaaa
       61 aaagcaggct ctatgtctag tccagatgat tttgaaactg caccagcaga atatgttgat
      121 gcattggatc cgagtatggt cgttgttgac agtggttctg ctgcagtcac agcgccatcg
      181 gattcagccg ctgaggtaag agctaatcaa aacgaagaaa acactggtgc tactgctgct
      241 gaaactagtg agaaagtgga tcagacggag gtcgaaaaga aagatgatga tgataccact
      301 gaagtcggtg taacaactac cacgccttcc attgctgata ctgctgctac cgctaacatt
      361 gcttcgacat caggtgcatc tgttactgaa cctactactg atgacaccgc tgctgatgag
      421 agaagagaac aagtaagtgg tcctcctctg tcaaatatga aattctatct taatcgcgac
      481 gcggatgcgc atgactcttt aaatgatatt gatcaattag cgcgtctgat tagagcaaac
      541 ggtggtgaag tgctagactc caagccaaga gaatcaaaag agaatgtttt catcgtgtca
      601 ccctataatc ataccaattt accaacggta actccgactt atattaaggc atgttgtcaa
      661 agcaactcgc tactgaacat ggaaaattat ttagtaccat acgataactt tagagaagtt
      721 gtcgatagta ggttgcagga ggaatcgcat tctaatggtg tagataatag caatagcaat
      781 agcgataaca gagattctat caggcccaga acagaaataa tctctacgaa tactaatggc
      841 gctacggaag actcaaccag cgaaaaagtt atggtagacg ctgaacaaca agctagatta
      901 caagagcaag ctcagctcct tcgtcaacat gtaagcagca ccgcatcaat cacaagcgga
      961 gggcacaatg acttggtcca gatcgaacaa cctcaaagag atacctccaa taataataat
     1021 agc
//
