LOCUS       sequence-165609-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: pCAG-CyRFP1
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..34
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             join(38..46,47..61)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             68..769
                     /codon_start=1
                     /product="cyan-excitable red fluorescent protein (Laviv et 
                     al., 2016)"
                     /label=CyRFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
ORIGIN
        1 attcgctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
       61 ggatccgatg gtgagcaagg gcgaggagct gatcaaggag aacatgagaa gcaagctgta
      121 cctggaaggc agcgtgaacg gccaccagtt caagtgcacc cacgaagggg agggcaagcc
      181 ctacgagggc aagcagacca acaggatcaa ggtggtggag ggaggccccc tgccgttcgc
      241 attcgacatc ctggccacca tgtttatgta cgggagcaag gtgttcatca agtaccccgc
      301 cgacctcccc gattatttta agcagtcctt ccctgagggc ttcacatggg agagagtcat
      361 ggtgttcgaa gacgggggcg tgctgaccgc cacccaggac accagcctcc aggacggcga
      421 gctcatctac aacgtcaagc tcagaggggt gaacttccca gccaacggcc ccgtgatgca
      481 gaagaaaaca ctgggctggg agcccagcac cgagaccatg taccccgctg acggcggcct
      541 ggaaggcaga tgcgacaagg ccctgaagct cgtgggcggg ggccacctgc acgtcaactt
      601 caagaccaca tacaagtcca agaaacccgt gaagatgccc ggcgtccact acgtggaccg
      661 cagactggaa agaatcaagg aggccgacaa cgagacctac gtcgagcagt acgagcacgc
      721 tgtggccaga tactccaacc tgggcggagg catggacgag ctgtacaagt ccggactcag
      781 atctcgagct caagcttcga attctgcagt cgacggtacc gcgg
//
