LOCUS       sequence-16561-e        1247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD18_32
FEATURES             Location/Qualifiers
     source          1..1247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             139..159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(249..271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gnngctgcgg gtcgactcta gaggatcccc ggggattggc caaaggaccc aaaggtatgt
       61 ttcgaatgat actaacataa catagaacat tttcaggagg acccttggag ggtaccgagc
      121 tcagaaaaaa tgactgctcc aaagaagaag cgtaaggtac cggtgggtga agaccagaaa
      181 cagcacctcg aactgagccg cgatattgcc cagcgtttca acgcgctgta tggcgagatc
      241 gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      361 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
      421 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc
      481 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc
      541 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca
      601 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt
      661 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt
      721 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg
      781 atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc
      841 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat
      901 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcanatg
      961 tgcggcgagt tgcgtgacta cctacgggta acagttttct ttatggcagg gtgaaacgca
     1021 ggtcgccagc ggcaccgcgc ctttcggngg tgaaattatc gatgancgtg gtggttatgc
     1081 cgatcgcgtc ccactacgtc tgaacgtcna aanccgaaac tggggagcnc cnaaatcccg
     1141 aatttttttt cgngggggng gttgaantgc caccncccaa ggnacnntta ttgaaacaaa
     1201 ancntgcnat ntncggtttt cccngagggg ggnatttaaa aggntct
//
