LOCUS       sequence-165613-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: pCAG-CyRFP1
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(61..762)
                     /codon_start=1
                     /product="cyan-excitable red fluorescent protein (Laviv et 
                     al., 2016)"
                     /label=CyRFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     CDS             complement(join(769..783,784..792))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cgggcccgcg gtaccgtcga ctgcagaatt cgaagcttga gctcgagatc tgagtccgga
       61 cttgtacagc tcgtccatgc ctccgcccag gttggagtat ctggccacag cgtgctcgta
      121 ctgctcgacg taggtctcgt tgtcggcctc cttgattctt tccagtctgc ggtccacgta
      181 gtggacgccg ggcatcttca cgggtttctt ggacttgtat gtggtcttga agttgacgtg
      241 caggtggccc ccgcccacga gcttcagggc cttgtcgcat ctgccttcca ggccgccgtc
      301 agcggggtac atggtctcgg tgctgggctc ccagcccagt gttttcttct gcatcacggg
      361 gccgttggct gggaagttca cccctctgag cttgacgttg tagatgagct cgccgtcctg
      421 gaggctggtg tcctgggtgg cggtcagcac gcccccgtct tcgaacacca tgactctctc
      481 ccatgtgaag ccctcaggga aggactgctt aaaataatcg gggaggtcgg cggggtactt
      541 gatgaacacc ttgctcccgt acataaacat ggtggccagg atgtcgaatg cgaacggcag
      601 ggggcctccc tccaccacct tgatcctgtt ggtctgcttg ccctcgtagg gcttgccctc
      661 cccttcgtgg gtgcacttga actggtggcc gttcacgctg ccttccaggt acagcttgct
      721 tctcatgttc tccttgatca gctcctcgcc cttgctcacc atcggatcct tatcgtcatc
      781 gtcgtacaga tc
//
