LOCUS       sequence-165618-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyC-kan
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             114..131
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      190..236
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcaccaacc ctccaccggc cgcgctcgcg gccaccaccc gataagagaa agggagtcca
       61 ccatgggctc ggccggtacc ggatccgaat tcgatatcgc tagccaagct tcgcaccacc
      121 accaccacca ctgataacag cttatcgatg tcgacgtagt taactagcgt acgatcgact
      181 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatgcca
      241 tcatggccgc ggtgatcagc tagagggggc gctgattaga aaaactcatc gagcatcaaa
      301 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
      361 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
      421 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
      481 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaaga
      541 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
      601 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
      661 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
      721 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
      781 ttcccgggga tcgca
//
