LOCUS       sequence-165622-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fusion protein of RhoA, FRET donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: mCyRFP1-RhoA
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..748
                     /codon_start=1
                     /product="monomeric cyan-excitable red fluorescent protein 
                     (Laviv et al., 2016)"
                     /label=mCyRFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTA
                     RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KKLKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggagctg atcaaggaga acatgagaag caagctgtac ctggaaggca gcgtgaacgg
      121 ccaccagttc aagtgcaccc acgaagggga gggcaagccc tacgagggca agcagaccgc
      181 caggatcaag gtggtggagg gaggccccct gccgttcgca ttcgacatcc tggccaccat
      241 gtttatgtac gggagcaagg tgttcatcaa gtaccccgcc gacctccccg attattttaa
      301 gcagtccttc cctgagggct tcacatggga gagagtcatg gtgttcgaag acgggggcgt
      361 gctgaccgcc acccaggaca ccagcctcca ggacggcgag ctcatctaca acgtcaagct
      421 cagaggggtg aacttcccag ccaacggccc cgtgatgcag aagaaaacac tgggctggga
      481 gcccagcacc gagaccatgt accccgctga cggcggcctg gaaggcagat gcgacaagaa
      541 gctgaagctc gtgggcgggg gccacctgca cgtcaacttc aagaccacat acaagtccaa
      601 gaaacccgtg aagatgcccg gcgtccacta cgtggaccgc agactggaaa gaatcaagga
      661 ggccgacaac gagacctacg tcgagcagta cgagcacgct gtggccagat actccaacct
      721 gggcggaggc atggacgagc tgtacaagtc cggactcaga tctcgaggga tggctgccat
      781 ccggaagaaa ctggtgattg ttggtgatgg agcctgtgga aagacatgct tgctcatagt
      841 cttcagcaag gaccagt
//
