LOCUS       sequence-165628-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Direct FRET pair Fusion mCyRFP1-mMaroon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: mCyRFP1-mMaroon
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..749
                     /codon_start=1
                     /product="monomeric cyan-excitable red fluorescent protein 
                     (Laviv et al., 2016)"
                     /label=mCyRFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTA
                     RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KKLKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
ORIGIN
        1 gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg
       61 gcgaggagct gatcaaggag aacatgagaa gcaagctgta cctggaaggc agcgtgaacg
      121 gccaccagtt caagtgcacc cacgaagggg agggcaagcc ctacgagggc aagcagaccg
      181 ccaggatcaa ggtggtggag ggaggccccc tgccgttcgc attcgacatc ctggccacca
      241 tgtttatgta cgggagcaag gtgttcatca agtaccccgc cgacctcccc gattatttta
      301 agcagtcctt ccctgagggc ttcacatggg agagagtcat ggtgttcgaa gacgggggcg
      361 tgctgaccgc cacccaggac accagcctcc aggacggcga gctcatctac aacgtcaagc
      421 tcagaggggt gaacttccca gccaacggcc ccgtgatgca gaagaaaaca ctgggctggg
      481 agcccagcac cgagaccatg taccccgctg acggcggcct ggaaggcaga tgcgacaaga
      541 agctgaagct cgtgggcggg ggccacctgc acgtcaactt caagaccaca tacaagtcca
      601 agaaacccgt gaagatgccc ggcgtccact acgtggaccg cagactggaa agaatcaagg
      661 aggccgacaa cgagacctac gtcgagcagt acgagcacgc tgtggccaga tactccaacc
      721 tgggcggagg catggacgag ctgtacaagt ccggactcag atctcgagct atggtgagca
      781 agggcgagga gctgatcaag gagaacatgc acac
//
