LOCUS       sequence-165635-         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLIM acceptor for mEGFP Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Hedrick NG, Harward SC, Hall CE, Murakoshi H, McNamara JO, Yasuda R
  TITLE     Rho GTPase complementation underlies BDNF-dependent homo- and 
            heterosynaptic plasticity.
  JOURNAL   Nature. 2016 Sep 28;538(7623):104-108. doi: 10.1038/nature19784.
  PUBMED    27680697
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-09-28- 28"; volume: "538"; issue: "7623"; 
            pages: "104-108"
COMMENT     Sequence Label: mCherry-PAK2 binding domain
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(178..196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     446..465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     640..663
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gcaagaattc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggacaaca
       61 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      181 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      241 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      361 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      481 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
      541 tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gctggacatc acctcccaca
      661 acgaggacta cac
//
