LOCUS       sequence-165636-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLIM acceptor for mEGFP Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Hedrick NG, Harward SC, Hall CE, Murakoshi H, McNamara JO, Yasuda R
  TITLE     Rho GTPase complementation underlies BDNF-dependent homo- and 
            heterosynaptic plasticity.
  JOURNAL   Nature. 2016 Sep 28;538(7623):104-108. doi: 10.1038/nature19784.
  PUBMED    27680697
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-09-28- 28"; volume: "538"; issue: "7623"; 
            pages: "104-108"
COMMENT     Sequence Label: mCherry-PAK2 binding domain
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..716)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(86..109)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(284..303)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     553..571
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gccgcttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgctcg
       61 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt gatgttgacg
      121 ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcggcg
      181 tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg
      241 tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg
      301 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc
      361 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
      421 tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc
      481 ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc
      541 agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc
      601 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
      661 aagcgcatga actccttgat gatggccatg ttgtcctcct cgcccttgct caccattccg
      721 gatcccttct gctccaactt agtgatattt gatgtctgaa gcaagcgggc ccactgctct
      781 g
//
