LOCUS       sequence-165639-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GA-inducible KRAB-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE
            PGK...
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agaattcgcc accatggctg cgagcgatga agttaatctt attgagagca gaacagtggt
       61 tcctctcaat acatgggttt taatatccaa cttcaaagta gcctacaata tccttcgtcg
      121 ccctgatgga acctttaacc gacacttagc tgagtatcta gaccgtaaag tcactgcaaa
      181 cgccaatccg gttgatgggg ttttctcgtt cgatgtcttg attgatcgca ggatcaatct
      241 tctaagcaga gtctatagac cagcttatgc agatcaagag caacctccta gtattttaga
      301 tctcgagaag cctgttgatg gcgacattgt ccctgttata ttgttcttcc atggaggtag
      361 ctttgctcat tcttctgcaa acagtgccat ctacgatact ctttgtcgca ggcttgttgg
      421 tttgtgcaag tgtgttgttg tctctgtgaa ttatcggcgt gcaccagaga atccataccc
      481 ttgtgcttat gatgatggtt ggattgctct taattgggtt aactcgagat cttggcttaa
      541 atccaagaaa gactcaaagg tccatatttt cttggctggt gatagctctg gaggtaacat
      601 cgcgcataat gtggctttaa gagcgggtga atcgggaatc gatgttttgg ggaacattct
      661 gctgaatcct atgtttggtg ggaatgagag aacggagtct gagaaaagtt tggatgggaa
      721 atactttgtg acggttagag accgcgattg gtactggaaa gcgtttttac ccgagggaga
      781 agatagagag catccagcgt gtaatccgtt tagcccgaga gggaaaagct ta
//
