LOCUS       sequence-165642-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GA-inducible KRAB-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE
            PGK...
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   71..133
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(350..370)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(377..397)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(410..436)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(416..442)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 caattaaccc tcactaaagg gaacaaaagc tggattaatt aagcggccgc atacgcgtat
       61 actagattaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
      121 aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga
      181 tattaagttt tattatattt acacttacat actaataata aattcaacaa acaatttatt
      241 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
      301 aacttttaac tagctcgagt catccgttgc tcccactacc aatgccgtcc actttccgct
      361 ttttcttagg atcttccacc ttgcgttttt tcttggggga gccgctgccc aggctggcat
      421 aatcgggcac gtcataggga taggcgtcgc ctcccagctg agacaggtcg atccgtgtct
      481 cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctct ttggtgctgg
      541 tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat
      601 tggtcagggt aaacaggtgg atgatattct cggcctgctc tctgataggc ttgtctctgt
      661 gcttgttgta ggcgctcagc accttgtcca gattagcgtc ggccaggatc actctcttgg
      721 agaactcgct gatctgctcg atgatctcgt ccaggtagtg tttgtgctgt tccacaaaca
      781 gctgtttctg ctcattatcc tcgggggagc ccttcagctt ctcatagtgg ctggccaggt
      841 acaggaagtt cacatatttg gagggcaggg ccagttcgtt tcccttctgc agttcgccgg
      901 cagaggccag cattctcttc cggccgtttt ccagctcgaa cagggagtac ttaggcagct
      961 tgatgatcag gtcctttttc acttctttgt agcccttgg
//
