LOCUS       sequence-165654-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ABA-inducible VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40P...
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             591..740
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             747..767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagaattcgc caccatgggt gggggcgcgc caactcaaga cgaattcacc caactctccc
       61 aatcaatcgc cgagttccac acgtaccaac tcggtaacgg ccgttgctca tctctcctag
      121 ctcagcgaat ccacgcgccg ccggaaacag tatggtccgt ggtgagacgt ttcgataggc
      181 cacagattta caaacacttc atcaaaagct gtaacgtgag tgaagatttc gagatgcgag
      241 tgggatgcac gcgcgacgtg aacgtgataa gtggattacc ggcgaatacg tctcgggaga
      301 gattagatct gttggacgat gatcggagag tgactgggtt tagtataacc ggtggtgaac
      361 ataggctgag gaattataaa tcggttacga cggttcatag atttgagaaa gaagaagaag
      421 aagaaaggat ctggaccgtt gttttggaat cttatgttgt tgatgtaccg gaaggtaatt
      481 cggaggaaga tacgagattg tttgctgata cggttattag attgaatctt cagaaacttg
      541 cttcgatcac tgaagctatg aacgaattct caagcgctgc aggtactagt gatgctttag
      601 acgattttga cttagatatg cttggttcag acgcgttaga cgacttcgac ctagacatgt
      661 taggctcaga tgcattggac gacttcgatt tagatatgtt gggctccgat gccctagatg
      721 actttgatct agatatgcta ggtagtccca aaaagaagag gaaagtggga tcccagtatc
      781 tgcccgacac agatgataga caccgaatcg aagagaaacg caagcgaacg tatgaaacct
      841 tcaaatcgat catgaagaaa tcgcccttct cgggtccgac cgatcccagg cccccaccga
      901 gaaggattgc ggtcccgtcc cgctcgtcgg ccagcgtgcc gaagc
//
