LOCUS       sequence-165655-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ABA-inducible VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40P...
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             166..540
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(631..651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacgtgt
       61 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
      121 gaaaaacacg atgataatat ggccacaacc ggatccggag ctaccatggc caagttgacc
      181 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
      241 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
      301 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
      361 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
      421 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
      481 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
      541 gtttaaacca tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat
      601 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
      661 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
      721 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
      781 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
      841 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
      901 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
      961 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggat
//
