LOCUS       sequence-165656-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ABA-inducible VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40P...
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..21
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3..21
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   72..134
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(324..371)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gcaattaacc ctcactaaag ggaacaaaag ctggattaat taagcggccg catacgcgta
       61 tactagatta accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgcgt
      121 aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag
      181 atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat
      241 ttatgtttat ttatttatta aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca
      301 aaacttttaa ctagctcgag ttactttttc ttttttgcct ggccggcctt tttcgtggcc
      361 gccggccttt tgcccttttt gatgatctga gggtgcttct tagatttcac ttcatacagg
      421 ttgcccagaa tgtctgtgct gtacttctta atgctctggg tcttggaggc gattgtctta
      481 atgatcctgg ggggcctctt gtcgttcatg ttttccaggt actcgcggta ggtgatgtcg
      541 atcatgttca cttcgatccg gttcagcagg tcgttgttca cgccgatcac tctatacagc
      601 tcgccgttga tcttgatcag atcgttgttg tagaaggagg cgataaactc ggcctggttg
      661 ctgatcttct tcagcttctt agcttcctca tagcacttgc tattcacttc gtagtagttt
      721 tcttttttga tcacatccag attcttcacg gtcacgaact tgtacacgcc attgtccagg
      781 tacacgtcga atctgtaggg cttcagggac agcttcacga ccttgtttct gctgttgggg
      841 tagtcgtcgg tgatgtccag atgggcgttc agtttgttgc cgtaatactt aatcttcttg
      901 atcacggggc cgttgtcctt tttggagtac ttggtcaggt agttcccggt ttc
//
