LOCUS       sequence-165677-        8764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SOD1E133-Delta..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pLEX_307-SOD1E133-Delta
FEATURES             Location/Qualifiers
     source          1..8764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1797..2385
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1850..1870)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2268..2279)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2457..2690
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2768..2889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2805..2824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2859..2878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2929..3064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3084..3103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3085..3103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3113..3130)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3113..3129)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3122..3144)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3271..3726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3358..3377)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3568..3589
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3752..3856
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3857..3925,3926..4717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4075..4094)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4888..5476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5377..5396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5630..5647
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5764..5785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5800..5805,5806..5823,5824..5830)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5838..5854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5843..5865
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5862..5878
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5862..5878
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5897..5917
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5899..5917
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5945..6171
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6172..6352
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6399..6524
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7017..7250
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7435..7479
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7710..7920
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7732..7928
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7779..7914
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7960..8562
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7960..7979)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8456..8476
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8605..8722
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag cagctcagat ctcgagctca agcttcgaat tccatggcga
     1321 cgaaggccgt gtgcgtgctg aagggcgacg gcccagtgca gggcatcatc aatttcgagc
     1381 agaaggaaag taatggacca gtgaaggtgt ggggaagcat taaaggactg actgaaggcc
     1441 tgcatggatt ccatgttcat gagtttggag ataatacagc aggctgtacc agtgcaggtc
     1501 ctcactttaa tcctctatcc agaaaacacg gtgggccaaa ggatgaagag aggcatgttg
     1561 gagacttggg caatgtgact gctgacaaag atggtgtggc cgatgtgtct attgaagatt
     1621 ctgtgatctc actctcagga gaccattgca tcattggccg cacactggtg gtccatgaaa
     1681 aagcagatga cttgggcaaa ggtggaaatg aaagtacaaa gacaggaaac gctggaagtc
     1741 gtttggcttg tggtgtaatt gggatcgccc aatgaactag tacgcgttaa gtcgacaatc
     1801 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1861 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1921 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag cagttgtggc
     1981 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2041 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2101 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2161 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     2221 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2281 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2341 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     2401 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     2461 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     2521 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2581 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2641 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     2701 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     2761 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2821 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2881 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     2941 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3001 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3061 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     3121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3241 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     3301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3481 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3661 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3721 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3961 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4021 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4081 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4141 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4201 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4261 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4321 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4381 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4441 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4501 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4561 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4621 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4681 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5461 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5521 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5581 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5641 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5701 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     5761 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     5821 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     5881 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     5941 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     6001 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     6061 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     6121 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     6181 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6241 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6301 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     6361 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     6421 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     6481 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     6541 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     6601 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     6661 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     6721 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     6781 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     6841 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     6901 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     6961 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     7021 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     7081 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     7141 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     7201 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     7261 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     7321 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     7381 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     7441 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     7501 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     7561 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     7621 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     7681 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     7741 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7801 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7861 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7921 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     7981 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8041 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8101 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8161 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8221 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8281 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8341 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8401 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     8461 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8521 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     8581 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     8641 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     8701 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     8761 ccgg
//
