LOCUS       sequence-165680-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the dehydrogenase-deficient mutant 
            DLD-H450A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pPHAGE_DLD-H450A-C-TAP
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     112..131
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        112..130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    160..184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accggcggcc gcagatctct cgaggttgat ctaatacgac
      121 tcactatagg gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagtt
      181 ggcatgcaga gctggagtcg tgtgtactgc tccttggcca agagaggcca tttcaatcga
      241 atatctcatg gcctacaggg actttctgca gtgcctctga gaacttacgc agatcagccg
      301 attgatgctg atgtaacagt tataggttct ggtcctggag gatatgttgc tgctattaaa
      361 gctgcccagt taggcttcaa gacagtctgc attgagaaaa atgaaacact tggtggaaca
      421 tgcttgaatg ttggttgtat tccttctaag gctttattga acaactctca ttattaccat
      481 atggcccatg gaaaagattt tgcatctaga ggaattgaaa tgtccgaagt tcgcttgaat
      541 ttagacaaga tgatggagca gaagagtact gcagtaaaag ctttaacagg tggaattgcc
      601 cacttattca aacagaataa ggttgttcat gtcaatggat atggaaagat aactggcaaa
      661 aatcaagtca ctgctacgaa agctgatggc ggcactcagg ttattgatac aaagaacatt
      721 cttatagcca cgggttcaga agttactcct tttcctggaa tcacgataga tgaagataca
      781 atagtgtcat ctacaggtgc tttatcttta aaaaaagttc cagaaaagat ggttgttatt
      841 ggtgcaggag taataggtgt agaattgggt tcagtttggc aaagacttgg tgcagatgtg
      901 acagcagttg aatttttagg tcatgtaggt ggagttggaa ttgatatgga gatatctaaa
      961 aactttcaac gcatccttca aaa
//
