LOCUS       sequence-165683-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses direct fusion KRAB-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-Sp...
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..620
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(21..40)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     517..537
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(711..731)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aagaattcgc caccatgacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
       61 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
      121 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
      181 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
      241 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
      301 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
      361 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
      421 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
      481 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
      541 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
      601 cccgcaagcc cggtgcctga gtttaaacca tgcaagcttg atatcaagct tatcgataat
      661 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
      721 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
      781 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
      841 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac cccca
//
