LOCUS       sequence-165684-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses direct fusion KRAB-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-Sp...
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..270
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             298..996
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttgtcgacgc caccatggat
       61 gctaagtcac taactgcctg gtcccggaca ctggtgacct tcaaggatgt atttgtggac
      121 ttcaccaggg aggagtggaa gctgctggac actgctcagc agatcgtgta cagaaatgtg
      181 atgctggaga actataagaa cctggtttcc ttgggttatc agcttactaa gccagatgtg
      241 atcctccggt tggagaaggg agaagagccc catatgtcaa gcgctgcagg tgctaccatg
      301 agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac
      361 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
      421 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc
      481 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
      541 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
      601 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
      661 agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag
      721 gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc
      781 ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag
      841 aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
      901 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
      961 tgcgacctcc ctagcaaact ggggcacaag cttaattcaa gcgctgcagg
//
