LOCUS       sequence-165685-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the proteolysis-deficient mutant DLD-S456A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pPHAGE_DLD-S456A-C-TAP
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(149..175)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(149..175)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(191..214)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    246..270
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
       61 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
      121 aacgttaggg ggggggggcg gaattctagg cgtagtcggg cacgtcgtag gggtatcctc
      181 cagcggccga cttgtcatcg tcgtccttgt agtcctcgag ttcgaaccgc gggccctcta
      241 gatcaaccac tttgtacaag aaagttgggc aaaagttgat tgatttgcca aatgacgcag
      301 caagatttgc ttctctaaaa gcttcagcta aggtcggatg tgcatgacag actctagcta
      361 tatcttcaca ggatgctcca tattccaaag caagagcagc ttcatttacc atttctccag
      421 cacctggtcc aagaatatgt gctcccagta ctctgtctgt cgatttctgc ccaaggatct
      481 tcaccatgcc atctgtgtca gcatttgtct tagctctgct gttagcagca aatgggaatt
      541 tcccaacttt gtactcaata ccctcttctt tcaactgctc ttctgatttg ccaacccaag
      601 caacttcagg gtgtgtgtaa atcactgatg gcacacaatt gta
//
