LOCUS       sequence-165687-        8766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repressilator with integrated reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J
  TITLE     Synchronous long-term oscillations in a synthetic gene circuit.
  JOURNAL   Nature. 2016 Oct 12;538(7626):514-517. doi: 10.1038/nature19841.
  PUBMED    27732583
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-10-12- 12"; volume: "538"; issue: "7626"; 
            pages: "514-517"
COMMENT     Sequence Label: pLPT20
FEATURES             Location/Qualifiers
     source          1..8766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(712..806)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(836..1627,1628..1696))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1459..1478
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1697..1801)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(1940..2026)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2056..2769)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2893..2910)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2893..2909)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2928..3014)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3078..3791)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(3078..3791)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             3800..3811
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(3821..3846,3847..3852,3853..3869,3870..3875,
                     3876..3894))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    3851..3869
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3876..3894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3971..3989
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    4012..4028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4035..4051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4093..4104
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4111..4731
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4184..4203)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      4779..4865
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             4972..4983
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5001..6080
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5020..5039)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      6145..6231
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        join(6262..6280,6281..6286,6287..6303,6304..6309,
                     6310..6335)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    6262..6280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6287..6305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             6345..6356
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6363..7073
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     terminator      7145..7231
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7766..8716)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      join(8764..8766,1..220)
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ctagtcaatg ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct
       61 agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt
      121 tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa
      181 aaaagataaa aagaatagat cccagccctg tgtataactc actactttag tcagttccgc
      241 agtattacaa aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc
      301 ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct
      361 ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg
      421 gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg
      481 aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt
      541 ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca
      601 gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa
      661 ggcagcggta tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc
      721 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      781 cattactgga tctatcaaca ggagtccaag cgagctcgta aacttggtct gacagttacc
      841 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      901 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      961 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     1021 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1081 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1141 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1201 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1261 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1321 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1381 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1441 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1501 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1561 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1621 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1681 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1741 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1801 gcacatttcc ccgaaaagtg ccacctgacg tctctttcct gcgttatccc ctgattctgt
     1861 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc cagcctacac
     1921 cccgggtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     1981 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctctagactc
     2041 agctaattaa gcttatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
     2101 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gcttagattg
     2161 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
     2221 ttgataatgg tctgctagtt gaacgcctcc atcttcaatg ttgtgtctaa ttttgaagtt
     2281 agctttgatt ccattctttt gtttgtctgc cgtgatgtat acattgtctg agatgtagtt
     2341 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
     2401 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
     2461 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa
     2521 aaagtcatgc tgtttcatat gatctgggta tcttgcaaag cattgaacac cccaagtgag
     2581 agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag
     2641 ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtgccc
     2701 attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc
     2761 tttactcatg gatccatctc cttcttaaat gaattcaaat actgtccttc tagtgtagcc
     2821 gtagttaggc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2881 gagctcgaat tcactggccg tcgttttaca taggtctagg gcggcggatt tgtcctactc
     2941 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     3001 tttcgtttta tttgatgcct ctagcacgcg taccatggga tcccccgggc tgcaggaatt
     3061 cgatatcaag cttattattt gtatagttca tccatgccat gtgtaatccc agcagctgtt
     3121 acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagcttagat
     3181 tggtaggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt
     3241 tgttgataat ggtctgctag ttgaacacct ccatcttcaa tgttgtgtct aattttgaag
     3301 ttcgctttga ttccattctt ttgtttgtct gccgtgatgt atacattgtg tgagttatag
     3361 ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaaatcaat accttttaac
     3421 tcgattctat taacaagggt atcaccttca aacttgactt cagcacgtgt cttgtagttc
     3481 ccgtcatctt tgaaaaatat agttctttcc tgtacataac cttcgggcat ggcactcttg
     3541 aaaaagtcat gctgtttcat atgatctggg tatctcgcaa agcattgaac accataaccc
     3601 aaagtagtga caagtgttgg ccatggaaca ggtagttttc cagtagtgca aatcaattta
     3661 agggtaagtt ttccgtatgt tgcatcacct tcaccctctc cactgacaga aaatttgtgc
     3721 ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct
     3781 cctttactca tgcggtacct ttctcctctt taatgaattc ggtcagtgcg tcctgctgat
     3841 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactcgag
     3901 gtgaagacga aagggcctcg tgctcgagta cgtctaagaa accattatta tcatgacatt
     3961 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cacctcgaga attgtgagcg
     4021 gataacaatt gacattgtga gcggataaca agatactgag cacatcagca ggacgcactg
     4081 accgaattca ttaaagagga gaaaggtacc atgtccagat tagataaaag taaagtgatt
     4141 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
     4201 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     4261 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     4321 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     4381 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     4441 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     4501 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     4561 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     4621 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     4681 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc tgcagcaaac
     4741 gacgaaaact acgctttagc agcttaatct agaggcatca aataaaacga aaggctcagt
     4801 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     4861 caaatccgcc gccctagacc tagctgcagg tcgaggataa atatctaaca ccgtgcgtgt
     4921 tgactatttt acctctggcg gtgataatgg ttgcatgtac tagaattcat taaagaggag
     4981 aaaggtacca tatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     5041 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     5101 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     5161 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     5221 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     5281 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     5341 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     5401 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     5461 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     5521 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     5581 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     5641 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     5701 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     5761 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     5821 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     5881 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     5941 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     6001 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6061 cccgactgga aagcgggcag gcagcaaacg acgaaaacta cgctttagca gcttaaaagc
     6121 ttaattagct gagtctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     6181 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
     6241 tagacctagc tgcaggtcga gtccctatca gtgatagaga ttgacatccc tatcagtgat
     6301 agagatactg agcacatcag caggacgcac tgaccgaatt cattaaagag gagaaaggta
     6361 ccatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta
     6421 aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca
     6481 agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg
     6541 cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt
     6601 caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa
     6661 gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta
     6721 gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt
     6781 ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa
     6841 gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt
     6901 tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg
     6961 gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt
     7021 gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcgcagcaa
     7081 acgacgaaaa ctacgcttta gcagcttgat ctagaggatc ccatggtacg cgtgctagag
     7141 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     7201 gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagacctagg gtacgggttt
     7261 tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt tatcagaatc gcagatccgg
     7321 cttcaggttt gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac
     7381 gggaggcgtc actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg
     7441 tttcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc
     7501 atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc
     7561 atctgttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa aaactcgtaa
     7621 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc
     7681 ctttgatatc taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact
     7741 gatagataca agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta
     7801 gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca tgcttacttt
     7861 gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat
     7921 cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta
     7981 ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc
     8041 tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca
     8101 atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa
     8161 gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc
     8221 taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat
     8281 aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga
     8341 atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt
     8401 cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat
     8461 tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag
     8521 cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc
     8581 gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta
     8641 gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa
     8701 caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga
     8761 gatggg
//
