LOCUS       sequence-165716-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Cas9 and sgRNA scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Habashy et al
  TITLE     CRISPR/Cas9-based system for loss-of-function screening
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: lentiCRISPR v2-Blast
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      305..314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(333..544)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(606..681)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcttccagtc tgtggaagaa gctgtcgtcc accttggcca tctcgttgct gaagatctct
       61 tgcagatagc agatccggtt cttccgtctg gtgtatcttc ttctggcggt tctcttcagc
      121 cgggtggcct cggctgtttc gccgctgtcg aacagcaggg ctccgatcag gttcttcttg
      181 atgctgtgcc ggtcggtgtt gcccagcacc ttgaatttct tgctgggcac cttgtactcg
      241 tcggtgatca cggcccagcc cacagagttg gtgccgatgt ccaggccgat gctgtacttc
      301 ttgtccatgg tggcagcgct ctagaaccgg tcctgtgttc tggcggcaaa cccgttgcga
      361 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
      421 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
      481 ccggatcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct
      541 gcccactgac gggcaccgga gccaattccc actcctttca agacctagct agcgaattca
      601 aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattttaact
      661 tgctatttct agctctaaaa cagagacgta caaaaaagag caagaagcta aaaaagattt
      721 aaaaattatt tttagcgcag ttaatggaac aggaactaaa tttaccccaa aaatattacg
      781 tgaatcagga tataacgtta ttgaggttga agagcatgca tttgaagatg aaacatttaa
      841 aaatgttgta aatccaaatc cagaatttga tcctgcatga aaaataccgc ttgaatatgg
      901 tattaaacat gatgcagata ttattattat gaatgaccca gatgctgaca gatttggaat
      961 ggcaataaaa catgatggtc attttgtaag attagatgga aatcaaacag gaccaatttt
     1021 aattgattga aaattatcaa atctaa
//
