LOCUS       sequence-165718-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Cas9 and sgRNA scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Habashy et al
  TITLE     CRISPR/Cas9-based system for loss-of-function screening
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: lentiCRISPR v2-Blast
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    126..243
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        294..542
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     294..314
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     465..484
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
      121 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      181 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      241 tttcgggttt attacaggga cagcagagat ccagtttggt taattaaggt accgagggcc
      301 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
      361 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
      421 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
      481 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
      541 ccggagacgg ttgtaaatga gcacacaaaa tacacatgct aaaatattat attctatgac
      601 ctttataaaa tcaaccaaaa tcttcttttt aataacttta gtatcaataa ttagaatttt
      661 tatgttcctt tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg ctagttttag
      721 taactcgcgt tgttttcttc acctttaata atagctactc caccacttgt tcctaagcgg
      781 tcagctcctg cttcaatcat tttttgagca tcttcaaatg ttctaactcc accagctgct
      841 ttaactaaag cattgtcttt aacaactgac ttcattagtt taacatcttc aaatgttgca
      901 cctgattttg aaaatcctgt tgatgtttta acaaattcta atccagcttc aacagctatt
      961 tcacaagctt tcatgatttc ttcttttgtt aataaacaat tttccataat acattt
//
