LOCUS       sequence-165719-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses direct fusion VPR-Sa dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-Sad...
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..619
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(20..39)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     516..536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(710..730)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agaattcgcc accatgacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
       61 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
      121 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
      181 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
      241 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
      301 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
      361 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
      421 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
      481 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
      541 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
      601 ccgcaagccc ggtgcctgag tttaaaccat gcaagcttga tatcaagctt atcgataatc
      661 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
      721 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
      781 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
      841 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
      901 ggggcattgc caccacctgt ca
//
