LOCUS       sequence-165727-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses murine SRAP domain (250 aa) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
  TITLE     Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease.
  JOURNAL   Cell Rep. 2017 Oct 10;21(2):482-494. doi: 
            10.1016/j.celrep.2017.09.055.
  PUBMED    29020633
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.09"; journalName: "Cell Rep"; date: 
            "2017-10-10- 10"; volume: "21"; issue: "2"; pages: "482-494"
COMMENT     Sequence Label: pET30a-SRAP
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(46..93)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     89..107
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(160..177)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agggcgcgtc ccattcgcca atccggatat agttcctcct ttcagcaaaa aacccctcaa
       61 gacccgttta gaggccccaa ggggttatgc tagttattgc tcagcggtgg cagcagccaa
      121 ctcagcttcc tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc
      181 gagtggatgg aaggtgatat tgtctatggg gtggattagc ttcagagctt cctgagtggc
      241 gacctcacca aagtcgagcc atttggagac tgcttcttct ccatctagta tggcaggcat
      301 cctgctgtgg atgtcactca aacctctgca ggaatccaca gtgatgatgc tgtaggaata
      361 caggcactct ccccctggcg cttcccagca gtcaaagatc cctgccattg tcagcagcct
      421 ccagttgtcc cagacctttt ccttgttgtc agagctgtct gaagcatcgt tcccacctga
      481 cttctctgtc ttgatttgag gaaaatagat gaagtatggt tgcctctggt ttgttccctg
      541 acaccgctgc cactcgtaga atccatctgc taaaacaaca caccgccgtc ctttccccag
      601 aggaaccttg aatgactgct tctccattat ggtatcactg cgacagttgg tagtgttgaa
      661 ctgcagctta gaaggatcac tttctttgaa ccaagatggg actaagcccc atcgcatggg
      721 aataattatc cgatctgatg agtctgcatc cttctcaaag tgcagtctgg agagcagcac
      781 ggggctgctg gactgcgggc tcttgttgta ggaggggcag tacttgtcgg ggtccctcca
      841 ctgcgggagc cgccgccggc cctgccgatc ctgataggcg caggccctgg tgagaacctc
      901 tctgggcaag tgacaggacg ttcgcccgca cagatctggg ctg
//
