LOCUS       sequence-165729-        4974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SV2C , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SV2C_p.R121Q
FEATURES             Location/Qualifiers
     source          1..4974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2374..2473)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2490..2509)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2491..2509)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2514..2530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2514..2530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2961..3752
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3845..4433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4334..4353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4587..4604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4763..4790)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4882..4968)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggaagac tcttacaagg ataggacttc actgatgaag ggtgccaagg
      241 acattgccag agaggtgaag aaacaaacag taaagaaggt gaatcaagct gtggaccgag
      301 cccaggatga atacacccag aggtcctaca gtcggttcca agatgaagaa gatgatgatg
      361 actactaccc ggctggagaa acctataatg gtgaggccaa cgatgacgaa ggctcaagtg
      421 aagccactga ggggcatgat gaagatgatg agatctatga gggggagtat cagggcatcc
      481 ccagtatgaa ccaagcgaag gacagcatcg tgtcagtggg gcagcccaag ggcgatgagt
      541 acaaggaccg acaggagctg gaatcagaaa ggagagctga cgaggaagag ttagcccagc
      601 agtatgagct gataatccaa gaatgcggtc atggtcgttt tcagtgggcc cttttcttcg
      661 tcctgggcat ggctcttatg gcagacggtg tagaggtgtt tgtcgttggc ttcgtgttac
      721 ccagtgctga gacagacctc tgcatcccaa attcaggatc tggatggcta ggcagcatag
      781 tgtacctcgg gatgatggtg ggggcgttct tctggggagg actggcagac aaagtgggaa
      841 ggaaacagtc tcttctgatt tgcatgtctg tcaacggatt ctttgccttc ctttcttcat
      901 ttgtccaagg ttatggcttc tttctcttct gtcgcttact ttctggattc gggattggag
      961 gagccatacc cactgtgttc tcgtactttg ctgaagtcct ggcccgggaa aagcggggcg
     1021 aacacttgag ctggctctgc atgttctgga tgatcggtgg catctacgcc tctgccatgg
     1081 cctgggccat catcccgcac tacgggtgga gcttcagcat gggatcggcc taccagtttc
     1141 acagttggcg tgtgtttgtc atcgtctgtg cactcccctg tgtctcctcc gtggtggccc
     1201 tcacattcat gcctgaaagc ccacgatcct tgttggaggt tggaaaacat gatgaagctt
     1261 ggatgattct gaagttaatt catgacacca acatgagagc ccggggtcag cctgagaagg
     1321 tcttcacggt aaacaaaata aaaactccta aacaaataga tgagctgatt gaaattgaga
     1381 gtgacacagg aacatggtat aggaggtgtt ttgttcggat ccgcaccgag ctgtacggaa
     1441 tttggttgac ttttatgaga tgtttcaact acccagtcag ggataataca ataaagctta
     1501 caattgtttg gttcaccctg tcctttgggt actatggatt atccgtttgg ttccctgatg
     1561 tcattaaacc tctgcagtcc gatgaatatg cattgctaac cagaaatgtg gagagagata
     1621 aatatgcaaa tttcactatt aactttacaa tggaaaatca gattcatact ggaatggaat
     1681 acgacaatgg cagattcata ggggtcaagt tcaaatctgt aactttcaaa gactctgttt
     1741 ttaagtcctg cacctttgag gatgtaactt cagtgaacac ctacttcaag aactgcacat
     1801 ttattgacac tgtttttgac aacacagatt ttgagccata taaattcatt gacagtgaat
     1861 ttaaaaactg ctcgtttttt cacaacaaga cgggatgtca gattaccttt gatgatgact
     1921 atagtgccta ctggatttat tttgtcaact ttctggggac attggcagta ttgccaggga
     1981 acattgtgtc tgctctgctg atggacagaa ttgggcgctt aacaatgcta ggtggctcta
     2041 tggtgctttc ggggatcagc tgtttcttcc tttggttcgg caccagtgaa tccatgatga
     2101 taggcatgct gtgtctgtac aatggattga ccatctcagc ctggaactct cttgacgtgg
     2161 tcactgtgga actgtacccc acagaccgga gggcaacagg ctttggcttc ttaaatgcgc
     2221 tatgcaaggc agcagccgtc ctgggaaact taatatttgg ctctctggtc agcatcacca
     2281 aatcaatccc catcctgctg gcttctactg tgctcgtgtg tggaggactc gttgggctgt
     2341 gcctgcctga cacacgaacc caggttctga tgttgccaac tttcttgtac aaagttggca
     2401 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2461 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2521 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2581 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2641 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2701 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2761 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2821 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2881 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2941 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     3001 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     3061 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     3121 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3181 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3241 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3301 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3361 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3421 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3481 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3541 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3601 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3661 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3721 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3781 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3961 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4621 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4681 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     4741 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4801 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4861 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4921 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcct
//
