LOCUS       sequence-165732-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of 
            catalytic Cys2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
  TITLE     Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease.
  JOURNAL   Cell Rep. 2017 Oct 10;21(2):482-494. doi: 
            10.1016/j.celrep.2017.09.055.
  PUBMED    29020633
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.09"; journalName: "Cell Rep"; date: 
            "2017-10-10- 10"; volume: "21"; issue: "2"; pages: "482-494"
COMMENT     Sequence Label: pET30a-SRAP-delCys2
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..77)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     283..302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     390..409
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        390..408
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    409..433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             448..470
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             481..498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             508..525
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             532..576
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
       61 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
      121 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
      181 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      241 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      301 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      361 gatcgagatc gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc
      421 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg
      481 caccatcatc atcatcattc ttctggnctg gtgccacgcg gttctggtat gaaagaaacc
      541 gctgctgcta aattcgaacg ccagcacatg gacagcccag atctggggcg aacgtcctgt
      601 cacttgccca gagaggttct caccagggcc tgcgcctatc aggatcggca gggccggcgg
      661 cggctcccgc agtggaggga ccccgacaag tactgcccct cctacaacaa gagcccgcag
      721 tccagcagcc ccgtgctgct ctccagactg cactttgaga aggatgcaga ctcatcagat
      781 cggataatta ttcccatgcg atggggctta gtcccatctt ggttcaaaga aagtgatcct
      841 tctaagctgc agttcaacac taccaactgt cgcagtgata ccataatgga gaagcagtca
      901 ttcaaggttc ctctggggaa aggacggcgg tgtgttgttt tagcagatgg attctacgag
      961 tggcagcggt gtcagggaac aaaccagagg caaccatact
//
