LOCUS       sequence-165734-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses YedK in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
  TITLE     Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease.
  JOURNAL   Cell Rep. 2017 Oct 10;21(2):482-494. doi: 
            10.1016/j.celrep.2017.09.055.
  PUBMED    29020633
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.09"; journalName: "Cell Rep"; date: 
            "2017-10-10- 10"; volume: "21"; issue: "2"; pages: "482-494"
COMMENT     Sequence Label: p3XFlag-YedK
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..111
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             778..807
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(862..883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattaa ccatggacta caaagaccat
       61 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gctttgtgga
      121 cgctttgccc aatcccaaac gcgtgaagat taccttgcgc ttctcgcgga agatattgaa
      181 cgcgatattc cctatgatcc cgaacccatt ggcagataca acgtcgcgcc gggaaccaaa
      241 gtcctactgc tcagtgaacg tgatgaacac cttcatctgg atccggtttt ctggggatat
      301 gctcccggat ggtgggataa accgccgctg attaacgccc gcgtagaaac tgcggcaacc
      361 agtcgtatgt ttaaaccgct ctggcaacat ggtcgggcaa tctgttttgc cgatggctgg
      421 tttgagtgga aaaaagaagg cgacaaaaaa cagccttttt ttatctatcg tgctgatgga
      481 caacctattt ttatggccgc gataggtagc acaccatttg agcgtggtga cgaagccgaa
      541 ggatttttga tcgtcactgc tgcggcagat caaggtctgg tagatattca tgaccgccgc
      601 ccactggtac tgtcgccaga agctgcgcgg gaatggatgc ggcaagagat tagcggtaaa
      661 gaagcctcag aaatcgcggc cagtggctgt gttccagcaa accagttctc ctggcacccg
      721 gtatcgcgcg cggtgggtaa tgttaaaaac cagggcgcgg agttaattca acctgttgaa
      781 caaaaactca tctcagaaga ggatctgtag aattcaatgg tgagcaaggg cgaggagctg
      841 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      901 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      961 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     1021 ctgcagtgct tcgcccgc
//
