LOCUS       sequence-16574-e        1283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Glover JR, Kowal AS, Schirmer EC, Patino MM, Liu JJ, Lindquist S
  TITLE     Self-seeded fibers formed by Sup35, the protein determinant of 
            [PSI+], a heritable prion-like factor of S. cerevisiae.
  JOURNAL   Cell 1997 May 30;89(5):811-9.
  PUBMED    9182769
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1997-05-30"; volume: "89(5)"; pages: "811-9"
COMMENT     Sequence Label: pJCSUP35(1-253)
FEATURES             Location/Qualifiers
     source          1..1283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             55..84
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             97..108
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(942..960)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      956..1003
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gggnattccc tctgaatatt ttgtttaact ttaagaagga gatataccat gggccatcat
       61 catcatcatc atcatcatca tcacagcagc ggccatatcg aaggtcgtca tatgtcggat
      121 tcaaaccaag gcaacaatca gcaaaactac cagcaataca gccagaacgg taaccaacaa
      181 caaggtaaca acagatacca aggttatcaa gcttacaatg ctcaagccca acctgcaggt
      241 gggtactacc aaaattacca aggttattct gggtaccaac aaggtggcta tcaacagtac
      301 aatcccgacg ccggttacca gcaacagtat aatcctcaag gaggctatca acagtacaat
      361 cctcaaggcg gttatcagca gcaattcaat ccacaaggtg gccgtggaaa ttacaaaaac
      421 ttcaactaca ataacaattt gcaaggatat caagctggtt tccaaccaca gtctcaaggt
      481 atgtctttga acgactttca aaagcaacaa aagcaggccg ctcccaaacc aaagaagact
      541 ttgaagcttg tctccagttc cggtatcaag ttggccaatg ctaccaagaa ggttggcaca
      601 aaacctgccg aatctgataa gaaagaggaa gagaagtctg ctgaaaccaa agaaccaact
      661 aaagagccaa caaaggtcga agaaccagtt aaaaaggagg agaaaccagt ccagactgaa
      721 gaaaagacgg aggaaaaatc ggaacttcca aaggtagaag accttaaaat ctctgaatca
      781 acacataata ccaacaatgc caatgttacc agtgctgatg ccttgatcaa ggaacaggaa
      841 gaagaagtgg atgacgaagt tgttaacgat ggatcccggg ccctagctaa ctgatccggc
      901 tgctancaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      961 ataacccctt ggggcctcta aacgggtctt gaggggnttt ttgctgaaag gaggaactat
     1021 atccgnatan ttcttgaana cnaanggcct cgngataccc tatttttaag gttatgncat
     1081 ganaaaatgg nttctnaaac gtcaggggnc cttttcggga aatggngcgg aaccctattg
     1141 ttttttttcn aaacatncaa aagttnccct ntgaanaaaa ccnggaaatg ntncataatt
     1201 ngnaaaggaa attgnnttnc nctttcggnc ccctttccnt ttnggnattn cctcggttng
     1261 cccccaaacn gggaaanaaa gtg
//
