LOCUS       sequence-165741-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses direct fusion VPR-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-Spd...
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             57..206
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             213..233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcttcctcat ctccgggcct ttcgacctgc agcccaagct tgtcgacgcc accatggatg
       61 ctttagacga ttttgactta gatatgcttg gttcagacgc gttagacgac ttcgacctag
      121 acatgttagg ctcagatgca ttggacgact tcgatttaga tatgttgggc tccgatgccc
      181 tagatgactt tgatctagat atgctaggta gtcccaaaaa gaagaggaaa gtgggatccc
      241 agtatctgcc cgacacagat gatagacacc gaatcgaaga gaaacgcaag cgaacgtatg
      301 aaaccttcaa atcgatcatg aagaaatcgc ccttctcggg tccgaccgat cccaggcccc
      361 caccgagaag gattgcggtc ccgtcccgct cgtcggccag cgtgccgaag cctgcgccgc
      421 agccctaccc cttcacgtcg agcctgagca caatcaatta tgacgagttc ccgacgatgg
      481 tgttcccctc gggacaaatc tcacaagcct cggcgctcgc accagcgcct ccccaagtcc
      541 ttccgcaagc gcctgcccca gcgcctgcac cggcaatggt gtccgccctc gcacaggccc
      601 ctgcgcccgt ccccgtgctc gcgcctggac cgccccaggc ggtcgctcca ccggctccga
      661 agccgacgca ggccggagag ggaacactct ccgaagcact tcttcaactc cagtttgatg
      721 acgaggatct tggagcactc cttggaaact cgacagaccc tgcggtgttt accgacctcg
      781 cgtcagtaga taactccgaa tttcagcagc ttttgaacca gggtatcccg gtcgcgccac
      841 atacaacgga gcccatgttg atggaatacc ccgaagcaat cacgagactt gtgacgggag
      901 cgcagcggcc tcccgatccc gcacccgcac ctttgggggc acctggcctc cctaacggac
      961 ttttgagcgg cgacgaggat ttctcctcca tcgccgatat gga
//
