LOCUS       sequence-165743-       10943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide with fluorescent Cyan reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Herold
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FgH1tUTCyan
FEATURES             Location/Qualifiers
     source          1..10943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2748..2767
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2812..2830
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        2852..2927
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2952..3351
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3999..4018
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4204..4824
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             4834..4887
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4888..4890,4891..4893,4894..5607)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4933..4954)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5194..5213)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5541..5562
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5635..6223
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5688..5708)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6106..6117)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6226..6242)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6227..6243)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6748..6928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6954..6971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6960..7184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7230..7658
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7317..7336)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7527..7548
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7667..7687)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7672..8001
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7852..7987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7914..7933
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8049..8096
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8115..8489
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8619..8740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8656..8675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8710..8729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8789..8805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8789..8805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8802..8824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8813..8829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8837..8843,8844..8861,8862..8867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8882..8903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9020..9037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9191..9779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9271..9290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9950..10741,10742..10810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10573..10592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10811..10915)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta accatggaat tcgaacgctg acgtcatcaa cccgctccaa
     2641 ggaatcgcgg gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc cctggcagga
     2701 agatggctgt gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc
     2761 tgggaaatca ccataaacgt gaaatgtctt tggatttggg aatcttataa gtccctatca
     2821 gtgatagaga ttcccggaga cggacgtctc ggttttagag ctagaaatag caagttaaaa
     2881 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttttaatt
     2941 aagggtgcag cggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     3001 ggcgagcgct gccacgtcag acgaagggcg caggagcgtt cctgatcctt ccgcccggac
     3061 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     3121 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     3181 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     3241 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     3301 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagttgcgg
     3361 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     3421 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     3481 tggggggagc gcacaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtaagg
     3541 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     3601 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     3661 tggggaccct gacgtgaagt ttgtcactga ctggagaact cgggtttgtc gtctggttgc
     3721 gggggcggca gttatgcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3781 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     3841 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     3901 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     3961 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     4021 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     4081 atttgggtca atatgtaatt ttcagtgtta gactagtaaa gcttctgcag gtcgactcta
     4141 gaaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacagg atctgaattc
     4201 accatgtcca gactggacaa gagcaaagtc atcaactctg ccttggagct cctgaatgaa
     4261 gttggcattg agggcttgac caccaggaag ctggcccaga agctgggtgt ggagcagcct
     4321 accctgtact ggcatgtgaa gaacaagagg gctctgcttg atgccctggc cattgagatg
     4381 ttggacaggc accacaccca cttctgccct ctggaagggg agtcctggca ggacttcctg
     4441 aggaacaatg ccaagagctt cagatgtgcc ttgctctccc accgggatgg tgccaaagtt
     4501 cacttgggca ccaggcctac agagaagcag tatgagaccc tggagaacca gctggcattc
     4561 ctgtgccaac aaggcttctc cctggaaaat gccttgtatg ccctctctgc tgtgggccac
     4621 ttcaccttgg gctgtgtgct ggaggaccag gagcaccaag ttgccaagga ggagagggag
     4681 acccccacca ctgactccat gccaccactg ctgcggcaag ctattgagtt gtttgaccac
     4741 caaggggctg agcctgcatt cctttttggc ctggaactga tcatctgtgg cctggaaaag
     4801 cagctgaaat gtgagtctgg ctctggatcc ggtgagggca gaggaagtct gctaacatgc
     4861 ggtgacgtcg aggagaatcc tggcccaatg gtgagcaagg gcgaggagct gttcaccggg
     4921 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc
     4981 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5041 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
     5101 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5161 ggctacgtcc aggagcgtac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5221 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5281 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc
     5341 tatatcaccg ccgacaagca gaagaacggc atcaaggccc acttcaagat ccgccacaac
     5401 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5461 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5521 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5581 ctcggcatgg acgagctgta caagtgatga caattcgata tcaagcttat cgataatcaa
     5641 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5701 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5761 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5821 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5881 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5941 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6001 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6061 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6121 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6181 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     6241 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6301 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6361 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6421 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     6481 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     6541 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     6601 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     6661 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     6721 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6901 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     6961 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7021 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     7081 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7141 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     7201 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     7261 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7321 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7381 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7441 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7501 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7561 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7621 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     7681 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7741 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7801 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7861 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7921 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     7981 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     8041 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     8101 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     8161 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     8221 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     8281 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     8341 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     8401 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     8461 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     8521 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     8581 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     8641 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8701 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     8761 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8821 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8881 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8941 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9001 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9061 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9121 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9181 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9241 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9301 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9361 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9421 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9481 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9541 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9601 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9661 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9721 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9781 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9841 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9901 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9961 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10021 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10081 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10141 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10201 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10261 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10321 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10381 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10441 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10501 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10561 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10621 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10681 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10741 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10801 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10861 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10921 ttccccgaaa agtgccacct gac
//
