LOCUS       sequence-165745-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AND gate ABA/GA-inducible VPR-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042."
COMMENT     Sequence Label: pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE
            P...
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             563..712
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             719..739
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccaactcaa gacgaattca cccaactctc ccaatcaatc gccgagttcc acacgtacca
       61 actcggtaac ggccgttgct catctctcct agctcagcga atccacgcgc cgccggaaac
      121 agtatggtcc gtggtgagac gtttcgatag gccacagatt tacaaacact tcatcaaaag
      181 ctgtaacgtg agtgaagatt tcgagatgcg agtgggatgc acgcgcgacg tgaacgtgat
      241 aagtggatta ccggcgaata cgtctcgaga gagattagat ctgttggacg atgatcggag
      301 agtgactggg tttagtataa ccggtggtga acataggctg aggaattata aatcggttac
      361 gacggttcat agatttgaga aagaagaaga agaagaaagg atctggaccg ttgttttgga
      421 atcttatgtt gttgatgtac cggaaggtaa ttcggaggaa gatacgagat tgtttgctga
      481 tacggttatt agattgaatc ttcagaaact tgcttcgatc actgaagcta tgaacgaatt
      541 ctcaagcgct gcaggtacta gtgatgcttt agacgatttt gacttagata tgcttggttc
      601 agacgcgtta gacgacttcg acctagacat gttaggctca gatgcattgg acgacttcga
      661 tttagatatg ttgggctccg atgccctaga tgactttgat ctagatatgc taggtagtcc
      721 caaaaagaag aggaaagtgg gatcccagta tctgcccgac acagatgata gacaccgaat
      781 cgaagagaaa cgcaagcgaa cgtatgaaac cttcaaatcg atcatgaaga aatcgccctt
      841 ctcgggtccg accgatccca ggcccccacc gagaaggatt gcggtcccgt cccgctcgtc
      901 ggccagcgtg ccgaagcctg cgccgcagcc ctaccccttc acgt
//
