LOCUS       sequence-165754-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Firefly luciferase under the control of NDUFS6 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Gandin V, Masvidal L, Hulea L, Gravel SP, Cargnello M, McLaughlan S,
            Cai Y, Balanathan P, Morita M, Rajakumar A, Furic L, Pollak M, Porco
            JA Jr, St-Pierre J, Pelletier J, Larsson O, Topisirovic I
  TITLE     nanoCAGE reveals 5' UTR features that define specific modes of 
            translation of functionally related MTOR-sensitive mRNAs.
  JOURNAL   Genome Res. 2016 May;26(5):636-48. doi: 10.1101/gr.197566.115. Epub 
            2016 Mar 16.
  PUBMED    26984228
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.197566.115"; journalName: "Genome Res"; date: "2016-05";
            volume: "26"; issue: "5"; pages: "636-48"
COMMENT     Sequence Label: pCDNA3 NDUFS6(5'UTR)-FF
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(177..195)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     gap             941..954
                     /estimated_length=14
     gap             990..1005
                     /estimated_length=16
ORIGIN
        1 tncnnnagnn ncactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccaaggg tcaaaggcca gcggcgcaaa atggaagacg
      121 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
      181 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
      241 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
      301 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
      361 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
      421 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
      481 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
      541 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
      601 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
      661 ngtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctannnnnn
      721 nacctnnnnn ngtggccctt ccgcatagaa ctgcctgcgt cagnnnctcg catgccagnn
      781 ntcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
      841 atcacggtnn nggaatgttt actacactcg gnnntttgnn ntgtggattt cgagtcgtct
      901 taatgtatnn nnntgannnn nnnntgnttt nacgatncct nnnnnnnnnn nnnnttcaaa
      961 nnnnnttgct agtaccaacc cnnnnnnnan nnnnnnnnnn nnnnnctctg attg
//
