LOCUS       sequence-16576-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Glover JR, Kowal AS, Schirmer EC, Patino MM, Liu JJ, Lindquist S
  TITLE     Self-seeded fibers formed by Sup35, the protein determinant of 
            [PSI+], a heritable prion-like factor of S. cerevisiae.
  JOURNAL   Cell 1997 May 30;89(5):811-9.
  PUBMED    9182769
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1997-05-30"; volume: "89(5)"; pages: "811-9"
COMMENT     Sequence Label: pJCSUP35(123-254)
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..68
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             81..92
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(557..575)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      571..618
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(663..681)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatattttgt ttactttaga aggagatata ccatgggcca tcatcatcat catcatcatc
       61 atcatcacag cagcggccat atcgaaggtc gtcatatgtc tttgaacgac tttcaaaagc
      121 aacaaaagca ggccgctccc aaaccaaaga agactttgaa gcttgtctcc agttccggta
      181 tcaagttggc caatgctacc aagaaggttg gcacaaaacc tgccgaatct gataagaaag
      241 aggaagagaa gtctgctgaa accaaagaac caactaaaga gccaacaaag gtcgaagaac
      301 cagttaaaaa ggaggagaaa ccagtccaga ctgaagaaaa gacggaggaa aaatcggaac
      361 ttccaaaggt agaagacctt aaaatctctg aatcaacaca taataccaac aatgccaatg
      421 ttaccagtgc tgatgccttg atcaaggaac aggaagaaga agtggatgac gaagttgtta
      481 acgatggatc ccgggcccta gctaactgat ccggctgcta acaaagcccg aaaggaagct
      541 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      601 gtcttgaggg gttttttgct gaaaggagga actatatccg gataattctt gaagacgaaa
      661 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      721 gtcaggtggc ac
//
