LOCUS       sequence-165764-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA targeting human APC around codon I1417.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Guernet A, Mungamuri SK, Cartier D, Sachidanandam R, Jayaprakash A, 
            Adriouch S, Vezain M, Charbonnier F, Rohkin G, Coutant S, Yao S, 
            Ainani H, Alexandre D, Tournier I, Boyer O, Aaronson SA, Anouar Y, 
            Grumolato L
  TITLE     CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and 
            Functional Analysis of Oncogenic Driver Mutations.
  JOURNAL   Mol Cell. 2016 Aug 4;63(3):526-38. doi: 
            10.1016/j.molcel.2016.06.017. Epub 2016 Jul 21.
  PUBMED    27453044
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.06.017"; journalName: "Mol Cell"; date: 
            "2016-08-4- 4"; volume: "63"; issue: "3"; pages: "526-38"
COMMENT     Sequence Label: sgAPC(B)
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     137..156
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        236..311
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        408..693
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 tgcatcgata caggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
       61 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
      121 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
      181 ggctttatat atcttgtgga aaggacgaaa caccgccatg cagtggaatg gtaaggtttt
      241 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      301 cgagtcggtg cttttttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      361 ttttagcgcg tgcgccaatt ctgcagacaa atggctctag aggtacccgt tacataactt
      421 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta
      481 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      541 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      601 aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag
      661 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
//
